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In all the experiments the microcosms were randomly assigned treatment combinations. To create microcosms with added leaf litter, we added 20, 10 mm disks punched with a \# 5 cork borer from senescent tulip poplar leaves collected in the fall of 2013 (collection described above). In the litter experiement, the leaves were were softened in deionized water for approximately 5 minutes prior to punching. In the leached litter and leached litter SOD experiment, the leaves were leached in approximately 10 L of deionized water in the dark for 72 h prior to punching. In the litter experiment, the nutrient enriched treatments were created by enriching the pond water added to those bottles with 300 $\mu$g L\textsuperscript{-1} of DIN in the form of NH\textsubscript{4}NO\textsubscript{3} and 30 $\mu$g L\textsuperscript{-1} of DIP in the form of PO\textsubscript{4}.  In the litter experiment and leached litter experiment, the microcosms were incubated in the dark, at room temperature and agitated gently on a rocker-shaker. In the leached litter SOD experiment, the microcosms were incubated in the dark at 15.5\textsuperscript{o} C in a INCUBATOR. Between sampling events (described below) 15 ml of the overlying water was removed from the bottles and the tops were removed to permit gas exchange.  In the litter experiment and the leached litter SOD experiment, the organic matter content of the microcosms at the beginning of the experiment was estimated from 5, 10 ml samples of the sediment slurry, and 5 samples of 20 randomly selected leaf discs. In the leached litter experiment the initial organic matter content of the sediment and leaves in the microcosms was estimated from 4 replicate samples of sediment and leaf discs, rather than 5. These organic matter samples were placed into pre-weighed crucibles, dried at 50\textsuperscript{o} C, and then ashed at 550\textsuperscript{o} C to determine the mass of organic matter. The initial C:N of the sediments and leaf discs in the leached litter experiment was determined from 2 replicate samples of 20 leaves dried at 50\textsuperscript{o} C and then analyzed as described below.