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\subsection{Unleached Litter Experiment} \subsection{Sediment Oxygen Demand}  The sediments added to the mesocosms microcosms in the litter experiment  had an estimated dry bulk density that ranged between 0.25 to 0.26 g ml\textsuperscript{-1} and an organic matter content between 12.7 and 12.9\%. Using this information, we estimate that the sediments in the mesocosms contained between 3.16 and 3.33 g of organic matter. The mesocosms that received leaf litter each received between 0.04 and 0.08 additional g of organic matter with the added leaf litter, which would be the equivelent of 11.3 to 22.7 g coarse organic matter m\textsuperscript{-2}. The sediments added to the microcosms in the leached litter experiment had  In the lake from which the sediments were collected we measured between 57.3 and 163.0 g coarse organic matter m\textsuperscript{-2}, so the mesocosms used in the experiment contained at most 40\% of the coarse organic matter of the lake. However in the mesocosms all of the leaf litter was added to the surface of the sediments, while a portion of the leaf litter in the lakes was buried within the sediment. The dissolved oxygen concentration of the overlying water in the bottles ranged from 80.09 to 275.80 $\mu mols$ O\textsubscript{2} L\textsuperscript{-1} with a median of 224.80 $\mu mols$ O\textsubscript{2} L\textsuperscript{-1}. The dissolved oxygen concentration varied significantly over the course of the incubation (p < 0.0001) and was the lowest on the initial day of the incubation (Fig. \ref{fig:DO_days}). Additionally, the bottles containing leaf litter had significantly lower dissolved oxygen concentrations (p = 0.002) but this was only evident on day 0 and day 2 of the incubation (Fig. \ref{fig:DO_days}).