Kaitlyn Peters edited BOD.tex  about 9 years ago

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\subsection{BOD Processing}  Removed At each sampling, the BOD bottles were removed  from incubation. incubation, and then approximately 68ml of water were drawn from the bottle. 2,  15 ml removed and transferred to samples were collected with  a glass syringe and placed in  10 ml glass septum topped vials. One  vialusing a glass syringe. This procedure  was done twice. The first vial was fixed and became T--0 sealed  and the second vial then fixed for Time 0 (T-0). The other sample  was placed on the rocker--shaker rocker-shaker  to be incubated incubate  in the dark for 5 five  hours. 3 ml The final water collected  was removed for bacterial, nutrient,  and placed into absorbence samples. The 3ml bacterial samples were stored in  a bacterial vial with ml of gluteraldehyde. Bacterial and vial, preserved in gluteraldehyde in the fridge. The  absorbance samples were stored in the fridge and refrigerator, while the  nutrient samples were kept in the freezer. Bottles were set-up on 9 June 2014 and sampled on days 1 (10 June 2014),3, 8, 15, and 22. SOD was calculated as the change in dissolved oxygen over time. Dissolved oxygen was determined using the Winkler's Titration method.   At the conclusion of the experiment, a sample for sediment C:N ratio and a sediment ergosterol sample were taken from each of the BOD bottles. In addition, in BOD bottles with CPOM, leaf disks were taken for C:N ratio samples and leaf ergosterol samples.