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Kaitlyn Peters edited BOD.tex
about 9 years ago
Commit id: 1953603b4d77f78e01c1233a41a9251e93ba740c
deletions | additions
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index d06553c..eb5d77f 100644
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\subsection{BOD Processing}
Removed At each sampling, the BOD bottles were removed from
incubation. incubation, and then approximately 68ml of water were drawn from the bottle. 2, 15 ml
removed and transferred to samples were collected with a
glass syringe and placed in 10 ml
glass septum topped vials. One vial
using a glass syringe. This procedure was
done twice. The first vial was fixed and became T--0 sealed and
the second vial then fixed for Time 0 (T-0). The other sample was placed on the
rocker--shaker rocker-shaker to
be incubated incubate in the dark for
5 five hours.
3 ml The final water collected was
removed for bacterial, nutrient, and
placed into absorbence samples. The 3ml bacterial samples were stored in a bacterial
vial with ml of gluteraldehyde. Bacterial and vial, preserved in gluteraldehyde in the fridge. The absorbance samples were stored in the
fridge and refrigerator, while the nutrient samples
were kept in the freezer. Bottles were set-up on 9 June 2014 and sampled on days 1 (10 June 2014),3, 8, 15, and 22. SOD was calculated as the change in dissolved oxygen over time. Dissolved oxygen was determined using the Winkler's Titration method.
At the conclusion of the experiment, a sample for sediment C:N ratio and a sediment ergosterol sample were taken from each of the BOD bottles. In addition, in BOD bottles with CPOM, leaf disks were taken for C:N ratio samples and leaf ergosterol samples.