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\subsection{Incubation of Unleached Litter}  We evaluated the effect of leaf litter on sediment oxygen demand and nutrient flux using laboratory microcosms made from 300 ml BOD bottles. The experiment consisted of two treatments crossed in a randomized factorial design, with each treatment combination replicated 4 times. The treatment combinations were sediments only with ambient nutrients, sediments plus leaf litter with ambient nutrients, sediments only plus elevated dissolved inorganic phosphorus (DIP) and elevated dissolved inorganic nitrogen (DIN), and sediment plus leaf litter with elevated DIP and DIN.   To set up the microcosms, we collected sediments from Lancer Park Pond near the littoral zone (approximately 10 m from shore) on May 29, 2014, using an Ekman dredge. Two dredge samples were collected from a depth of 0.4 m and another 2 dredge samples were collected from a depth of 1 m. The sediments from each location were combined and sieved through a 250 µm mesh net to remove macroinvertebrates and CPOM. The material retained by the nets was dried at 50\textsuperscript{o} C and ashed at 550\textsuperscript{o} C for 4 h to determine the  AFDM of the CPOM in the pond. The water for the microcosms was collected from the pond at 0.5 m on June 9, 2014 by submerging 1 L plastic bottles. The sieved sediment slurry was homogenized and allowed to settle overnight. The overlaying water was siphoned off and 100 ml of sediment was added to each of the 300 ml BOD bottles. The bottles were then carefully filled with approximately 200 ml of pond water (with or without added nutrients, see below), taking care not to disturb the sediments.Five, 10 ml samples of the sediment slurry were collected and placed into pre-weighed crucibles, dried at 50\textsuperscript{o} C, and then ashed at 550\textsuperscript{o} C. to estimate the organic matter content of the sediments in the microcosms.  The microcosms were randomly assigned treatments. treatment combinations.  To create microcosms with added leaf litter, we added 20, 10 mm disks punched from senescent tulip poplar leaves collected in the fall of 2013 (collection described above) with a \# 5 cork borer, to randomly selected microcosms. Prior to punching the leaf discs the leaves were softened in deionized water for approximately 5 minutes. Nutrient enriched treatments were created by enriching the pond water added to those bottles with 300 $\mu$g L\textsuperscript{-1} of DIN in the form of NH\textsubscript{4}NO\textsubscript{3} and 30 $\mu$g L\textsuperscript{-1} of DIP in the form of PO\textsubscript{4}. The BOD bottles microcosms  were incubated in the dark, at room temperature, on rocker-shakers at speed 8 temperature  and tilt 8. To encourage gas exchange in the bottles, the bottles were stored with 15ml of water removed. Thus, the total water was approximately 185ml.   The organic matter content agitated gently on a rocker-shaker. Between sampling (described below) 15 ml  of the leaf disks was determined by randomly selecting 5 senescent tulip poplar leaves that were collected from fall 2013. Each leaf was gently submerged in DI overlying  wateruntil it  was soft enough to core (about 5 min). A single leaf disk (10mm, #5 cork borer) was cut removed  from theleaf blade avoiding the midrib. The disk was placed into a pre-weighed crucible and dried at 50 degrees Celcius, then ashed at 550 degrees Celcius. CPOM treated BOD  bottles ultimately recieved 20 leaf disks. and the tops were removed to permit gas exchange.  An initial water analysis was performed on the collected water to give a nutrient baseline for The organic matter content of  the ambient replacement water. Dr. Dina Leech filtered 50 microcosms was estimated from 5, 10  ml samples  of the collected pond water through the GFF and utilized Hach test kits to measure nitrate, nitrite, ammonia, sediment slurry,  and orthophosphate levels in the ambient water. N was tested using Hach Test Kit N1-12 (cat# 14081-00) using the provided instructions. Detection limits 5 randomly selected leaf discs. These organic matter samples  were <8.8 mg/L* for Nitrate, <0.066mg/L for Nitrite, <0.2 mg/L for Ammonia, <0.2 mg/L for Orthophosphate. The nutrient enriched water that was used for nutrient treatment BOD bottles had a target of 300 \mu m NH4NO3 placed into pre-weighed crucibles, dried at 50\textsuperscript{o} C,  and 30 \mu m then ashed at 550\textsuperscript{o} C to determine the mass  of KH2PO4. organic matter.  *This number needs to be corrected