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There are two ways for observing autopoiesis. The first one is the autopoiesis of the each variable. Variables with more complexity than other have a positive autopoiesis reflecting more autonomy. Variables with low complexity than other have negative autopoiesis, reflecting less autonomy. Results of autopoiesis by variable in each subsystem can be seen in annex **. In general, variables in categories of very high and high complexity, have more autopoiesis resulting as more autonomous; that means they have more capacity of adaptation in front the changes of their environment wich is constituted by the other subsystem variables.  The second one form of determine autopoiesis is among variables of different subsystems. According subsystems, according  with the matter-energy flux in ecosystems. It is well-know that photosynthetic living beings depending of solar radiation and nutrients availability as the base for its metabolism process. Also, zooplankton depending of  grazing phytoplankton populations. However, for life sustaining living beings should be more autonomous than their environment. This means that the variables related to living systems should have a greater complexity than the variables related to their environment (Fernández et al., 2014). Our experiments carried out shows that...  It is important to highligh that there is a dependence gradient: Zooplanktonic Population->Phytoplaktonic Populations->solar radiation....