deletions | additions       diff --git a/Nuclear area predicts cell cycle phases.tex b/Nuclear area predicts cell cycle phases.tex  index 9bfd0ef..9e19f66 100644  --- a/Nuclear area predicts cell cycle phases.tex  +++ b/Nuclear area predicts cell cycle phases.tex 
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\subsection{Nuclear area predicts correlates with  cell cycle phases} In order to validate the use of the nuclear area to infer the cell cycle phases we imaged NIH/3T3 fibroblasts containing the Fucci system, a two colors fluorescent reporter that allow to distinguish G1 phase from S/G2/M phase \cite{Sakaue-Sawano2008}. We modified our HMM to infer the cell cycle phase from the Fucci signal (denoted $\phi_F$) by multiplying the emission probabilities of both colors (Figure \ref{fig:cellCycleFucci}A, Section \ref{sec:methods_fucci}) and independently inferred the cell cycle phase $\phi_N$ from the nuclear area (Figure \ref{fig:cellCycleFucci}B).   When comparing the two phases we found a reasonable agreement (Figure \ref{fig:cellCycleFucci}C). The two methods are very accurate close to divisions (the abrupt change in the signal at division contains a lot of information) but show more variations in the middle of the cycle.