Rosamaria Cannavo edited NIH 3T3 Fucci cell line.tex  about 9 years ago

Commit id: 03db35914330ca309e38483f24daea05f77e8ae4

deletions | additions      

       

\subsection{NIH 3T3 Fucci and \histone 2B-mCherry  cell line} Rosie: write writing  method section for the different cell lines NIH-3T3 Fucci cells and Histone 2B-mCherry were produced starting from lentiviral plasmids .... mKO2 / ..... mAG from () and from PGK-H2B-mCherry lentiviral plasmid (from Mark Mercola, Addgene plasmid # 21217) \cite{19352491} respectively.  Lentiviral particles were produced in HEK293T cells using envelope vector pMD2.G and packaging plasmid psPAX2 as previously described (Salmon & Trono, 2007). Cells were transduced with viral particle-containing supernatants according to standard procedures, and transduced cells were sorted by FACS.  Reference for PGK-H2B-mCherry lentiviral plasmid (from Mark Mercola, Addgene plasmid # 21217) \cite{19352491}