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\label{fig:5}  \textbf{Pan-neuronalknock-down of  DmCa\textsubscript{v}3 knockdown  increases sleep.} \\  \textbf{(a)} Sleep profiles of over two days of 12h:12h light-dark cycle cycles  (LD) and two days of continuous dark condition darkness  (DD). Pan-neuronal gal4 driven knockdown of  DmCa\textsubscript{v}3 RNAi flies (\emph{Elav\textgreater{}RNAi}, orange line, (\emph{elav$>$RNAi}, orange,  n=44) have increased increases  sleep compared to both gal4 control(\emph{elav-gal4/+}, black line, beyond that of the heterozygous Gal4 control (\emph{elav-Gal4/+}, black,  n=38) and the heterozygous  UAS control (\emph{UAS-DmCa\textsubscript{v}3 RNAi/+}, grey line, (\emph{UAS-RNAi/+}, grey,  n=42). Sleep is plotted  in 30 minute intervals was plotted. intervals.  White, black black,  and grey bar bars  denote light phase, dark phase phase,  and subjective light phase, respectively. ZT, zeitgeber time.  CT, circadian time.  \textbf{(b)} Quantification of average total  sleepamount  over two days of light-dark cycle cycles  (LD) and two days of continuous dark condition darkness  (DD). Data are represented presented  as mean means  $\pm$ s.e.m.  For statistical analysis, One-way s.e.m and analyzed via one-way  ANOVA followed by the  Tukey-HSD post hoc test was performed.  ***p$<$0.001. test.  ***\emph{p}$<$0.001