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Kyunghwa Jeong edited Results_expression.tex
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We then used the $\phi$C31 integrase to insert into the \emph{attP} landing site of \emph{DmCa\textsubscript{v}3\textsuperscript{Founder, \emph{w-}}} an \emph{attB}vector (\emph{pGE-attB\textsuperscript{GMR}}) containing the deleted genomic region plus an additional GFP coding sequence and linker sequence in-frame before the start codon of DmCa\textsubscript{v}3.
This produced the \emph{GFP::DmCa\textsubscript{v}3} line, which expresses an N-terminally GFP-tagged DmCa\textsubscript{v}3 under the control of its own endogenous promoter.
In these \emph{GFP::DmCa\textsubscript{v}3} flies, GFP fluorescence appears broadly across the brain
while control flies show little GFP signal (Fig.
\ref{fig:2}b). \ref{fig:2}b and \ref{fig:S1}).
\emph{GFP::DmCa\textsubscript{v}3} is expressed in well-structured neuropils like the antennal lobes, the mushroom bodies, the central complex (Fig. \ref{fig:2}c-h), the optic lobes, as well as in some of the less-structured neuropils.
The central complex---comprising the fan-shaped body, ellipsoid body, noduli, and protocerebral bridge---shows the strongest expression with the ventral fan-shaped body and ventral noduli particularly prominent (Fig. \ref{fig:2}e and g).
In mushroom body neurons, there is far more \emph{GFP::DmCa\textsubscript{v}3} in the dendrite-rich calyx of the dorso-posterior brain (Fig. \ref{fig:2}h) than the axon-rich lobes of the anterior brain (Fig. \ref{fig:2}d).