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The voltage-dependent kinetics of the three mammalian T-type calcium channels are known to differ, with Ca\textsubscript{v}3.1 and Ca\textsubscript{v}3.2 showing faster activation/inactivation kinetics than Ca\textsubscript{v}3.3\cite{klockner:1999aa}.
To compare the time constants of activation and inactivation for DmCa\textsubscript{v}3 and Ca\textsubscript{v}3.1, we fitted current traces with a double exponential function.
At test potentials ranging from \textminus50 mV to +20 mV, DmCa\textsubscript{v}3 has slower current kinetics than Ca\textsubscript{v}3.1 (\emph{p}$<$ 0.01 for $\tau$\textsubscript{act} and \emph{p}$<$ 0.001 for $\tau$\textsubscript{inact}, Student's t-test, Fig. \ref{fig:1}c).
For example, the activation and inactivation time constants of DmCa\textsubscript{v}3 current at a \textminus20 mV test potential are 2.2 $\pm$ 0.2 ms and 23.4 $\pm$ 1.4 ms respectively, whereas the activation and inactivation time constants of Ca\textsubscript{v}3.1 current at the same
test potential are 1.1 $\pm$ 0.2 ms and 9.7 $\pm$ 0.9 ms, respectively. This means the activation and inactivation kinetics of DmCa\textsubscript{v}3 are 2-fold slower than those of rat Ca\textsubscript{v}3.1, but still in the ``fast'' range (Table \ref{tab:1}).
Another defining property of the LVA T-type calcium channels is that they deactivate much more slowly than HVA calcium channels\cite{PerezReyes:1998gn,lee:1999aa,matteson:1986aa}.
To characterize the deactivation kinetics of DmCa\textsubscript{v}3, we performed a transient transfection of the DmCa\textsubscript{v}3 cDNA into HEK-293 cells followed by whole-cell patch clamp recordings of tail currents.