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Kyunghwa Jeong edited Results_sleep.tex
over 8 years ago
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Sleep bout length, on the other hand, is increased under both LD and DD conditions and rescued in \emph{DmCa\textsubscript{v}3\textsuperscript{Rescue}} flies (Fig. \ref{fig:3}g).
To confirm that this elevated sleep phenotype is specific to DmCa\textsubscript{v}3 loss-of-function, we generated three independent deletion mutants via imprecise P-element excision.
As expected, all three deletion mutants as well as a trans-heterozygous mutants ($\Delta$3/$\Delta$115) show increased sleep, especially in constant darkness (Fig.
\ref{fig:S2}). \ref{fig:S3}).
In addition, knockdown of DmCa\textsubscript{v}3 in its own neurons (\emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}$>${}UAS-DmCa\textsubscript{v}3-IR}) increases sleep after the third day of continuous darkness (Fig.
\ref{fig:S3}). \ref{fig:S4}).
Together, these results implicate DmCa\textsubscript{v}3 as a novel sleep-inhibitor of fly sleep.