Kyunghwa Jeong edited Results_sleep.tex  almost 9 years ago

Commit id: d56b9ff37cf83c00649bd1996b5ea0a62e0b3f5c

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To confirm that this elevated sleep phenotype is specific to DmCa\textsubscript{v}3 loss-of-function, we generated three independent deletion mutants via imprecise P-element excision.  As expected, all three deletion mutants as well as a trans-heterozygous mutants ($\Delta$3/$\Delta$115) show increased sleep, especially in constant darkness (Fig. \ref{fig:S2}).  In addition, knockdown of DmCa\textsubscript{v}3 in its own neurons (\emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}$>$UAS-DmCa\textsubscript{v}3-IR}) (\emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}\textgreater{}UAS-DmCa\textsubscript{v}3-IR})  increases sleep after the third day of continuous darkness (Fig. \ref{fig:S3}). Together, these results implicate DmCa\textsubscript{v}3 as a novel sleep-inhibitor of fly sleep.