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\subsection*{DmCa\textsubscript{v}3 channel produces LVA currents in \emph{Xenopus} oocytes}  We cloned a full length fly T-type Ca\textsuperscript{2+} channel cDNA (named DmCa\textsubscript{v}3) from a fly head cDNA library. \textred{Which one?}  The sequence of cloned cDNA was exactly matched to an isoform predicted in FlyBase (\href{http://}{http://flybase.org}).   To examine the electrophysiological properties of DmCa\textsubscript{v}3, we injected cRNAs made from the DmCa\textsubscript{v}3 cDNA template into \emph{Xenopus} oocytes. 
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Application of low micro-molar concentrations of Ni\textsuperscript{2+} solutions inhibited DmCa\textsubscript{v}3 currents in a concentration dependent manner (Fig. \ref{fig:1}f).  In contrast, Ca\textsubscript{v}3.1 currents required much higher concentrations of Ni\textsuperscript{2+} solutions to be blocked (Fig. \ref{fig:1}f).  Analysis of dose-response curves shows that the IC\textsubscript{50} values for DmCa\textsubscript{v}3 and Ca\textsubscript{v}3.1 are 5.12 and 276.5 $\mu$M, respectively.  These results indicate that the Ni\textsuperscript{2+} block of DmCa\textsubscript{v}3 currents is ~50 fold more sensitive than that of Ca\textsubscript{v}3.1 , suggesting that the nickel sensitive block of DmCa\textsubscript{v}3 channel resembles Ca\textsubscript{v}3.2 T-type channel (Table \ref{tab:1}).