Kyunghwa Jeong edited Results_clock.tex  almost 9 years ago

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As in other animals, sleep in \emph{Drosophila} is regulated by the circadian clock, meaning clock mutants generally show altered sleep phenotypes\cite{hendricks:2003aa, parisky:2008aa}.  We therefore asked whether the increased sleep observed in DmCa\textsubscript{v}3-null flies can be attributed to a disrupted circadian clock.  After monitoring their activity over seven days of continuous darkness, we found that \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} flies have a slightly elongated period length (24.3 $\pm$ 0.6 vs. 23.9 $\pm$ 0.2), a reduced power of rhythmicity (22.3 $\pm$ 2.9 vs. 53.4 $\pm$ 5.1), and reduced overall percentage of rhythmic flies (70.3\% vs. 92.6\%) when compared to \emph{w\textsuperscript{1118}} (Fig. \ref{fig:4}a).  This circadian phenotype reduced activity is  probably due to the increased sleep at the transition of subjective day and subjective night. We also checked whether the transcriptional oscillation of \emph{period}, one of the core clock genes, is normal in \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}}.   Period gene in \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} showed rhythmic circadian mRNA levels in continuous darkness, peaked at CT12 as \emph{w\textsuperscript{1118}} control (Fig. \ref{fig:4}b).  These robust rhythmic behavior in free-running period and rhythmic oscillation of clock gene suggest sleep phenotype of result suggests that  \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} is mutant does  not due to affect molecular clock although  the defect behavioral output is somewhat altered both  in the circadian system. sleep and daily locomotion rhythm.