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Kyunghwa Jeong edited Results_clock.tex
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Sleep is regulated by circadian system and some clock mutants show altered sleep phenotype\cite{12568241, 19038223}.
We examined whether this increased sleep phenotype is due to altered circadian clock.
Activity of
DmCa\textsubscript{v}3\textsuperscript{Gal4} \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} was monitored for
more than seven days of continuous
darkness (Fig. \ref{fig:4}A).
DmCa\textsubscript{v}3\textsuperscript{Gal4} darkness.
\emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} flies showed
somewhat decreased, but significant rhythmic activity
rhythms (70.6\% rhythmicity) in until the 7th day of continuous
darkness dark period although they have increased period length and reduced rhythmicity compared to
w\textsuperscript{1118} background control (90.7\% rhythmicity).
Decreased emph{w\textsuperscript{1118}} (Fig. \ref{fig:4}a).
The reduced percentage of rhythmic flies(90.7\% rhythmicity) and power of
DmCa\textsubscript{v}3\textsuperscript{Gal4} rhythmicity (24.0 $\pm$ 3.4)
compared in \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} are probably due to
w\textsuperscript{1118} (61.4 $\pm$ 6.5) reflect the increased sleep at the transition of subjective day and
night period.
Consistent with circadian locomotion, transcription level subjective night.
We also checked whether the transcriptional oscillation of
period which is \emph{period}, one of the clock genes
showed is normal
circadian oscillation in
DmCa\textsubscript{v}3\textsuperscript{Gal4} \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}}.
Period gene in
\emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} showed rhythmic circadian mRNA levels during continuous
dark period darkness, peaked at CT12 as emph{w\textsuperscript{1118}} control (Fig.
\ref{fig:4}B). \ref{fig:4}b).
These
data robust rhythmic behavior in free-running period and rhythmic oscillation of clock gene suggest sleep phenotype of
DmCa\textsubscript{v}3 mutant are \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} is not due to
altered clock the defect in the circadian system.