Kyunghwa Jeong edited Results_clock.tex  almost 9 years ago

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Sleep is regulated by circadian system and some clock mutants show altered sleep phenotype\cite{12568241, 19038223}.  We examined whether this increased sleep phenotype is due to altered circadian clock.  Activity of DmCa\textsubscript{v}3\textsuperscript{Gal4} \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}}  was monitored formore than  seven days of continuous darkness (Fig. \ref{fig:4}A).  DmCa\textsubscript{v}3\textsuperscript{Gal4} darkness.  \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} flies  showed somewhat decreased, but significant rhythmic  activity rhythms (70.6\% rhythmicity) in until the 7th day of  continuous darkness dark period although they have increased period length and reduced rhythmicity  compared to w\textsuperscript{1118} background control (90.7\% rhythmicity).  Decreased emph{w\textsuperscript{1118}} (Fig. \ref{fig:4}a).   The reduced percentage of rhythmic flies(90.7\% rhythmicity) and  power of DmCa\textsubscript{v}3\textsuperscript{Gal4} rhythmicity  (24.0 $\pm$ 3.4) compared in \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} are probably due  to w\textsuperscript{1118} (61.4 $\pm$ 6.5) reflect the  increased sleep at the transition of subjective day and night period.  Consistent with circadian locomotion, transcription level subjective night.  We also checked whether the transcriptional oscillation  of period which is \emph{period},  one of the clock genes showed is  normalcircadian oscillation  in DmCa\textsubscript{v}3\textsuperscript{Gal4} \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}}.   Period gene  in \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} showed rhythmic circadian mRNA levels during  continuous dark period darkness, peaked at CT12 as emph{w\textsuperscript{1118}} control  (Fig. \ref{fig:4}B). \ref{fig:4}b).  These data robust rhythmic behavior in free-running period and rhythmic oscillation of clock gene  suggest sleep phenotype of DmCa\textsubscript{v}3 mutant are \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} is  not due to altered clock the defect in the circadian  system.