Kyunghwa Jeong edited Methods_sleep.tex  almost 9 years ago

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5 minutes and more inactivity were counted as sleep conventionally.  After one day of habituation in the incubator, sleep during two days of 12hr:12hr light-dark cycle and following two days of dark period was analysed.  Circadian locomotion was measured every 30 min bin and analysed using ClockLab analysis software (Actimetrics) with the significance level of the $\Xi$2 $\chi$\textsuperscript{2}  periodogram set to a = 0.05. Flies with a $\Xi$2 statistic $\geq$10 over the significance line were scored as rhythmic.