deletions | additions       diff --git a/Methods_electrophys.tex b/Methods_electrophys.tex  index ad2727b..59b8aa1 100644  --- a/Methods_electrophys.tex  +++ b/Methods_electrophys.tex 
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Data for tail currents were filtered at 10 kHz and digitized at 20 kHz.   Peak currents and exponential fits to currents were analyzed using Clampfit software (Axon instruments, Foster City, CA, USA).   Activation and inactivation time constants of T-type channel currents elicited by step pulses were estimated by fitting individual current traces with a double exponential function: $A1(1-exp(-t/\tau1)) + A2(1-exp(-t/\tau2))$ where $A1$ and $A2$ are the coefficients for the activation and inactivation exponentials, $t$ is time, and $\tau1$ and $\tau2$ are the activation and inactivation time constants, respectively.  The smooth curves for channel activation and steady-state inactivation were from fitting data with a Boltzmann equation: $\{1+exp[(V_{50}-V)/S_{act}]\}^{-1}$ , $\{1+exp[(V_{50}-V)/S_{act}]\}^{-1}$,  where $V_{50}$ is the potential for half-maximal activation and $S_{act}$ is the slope conductance. Dose-response curves for Ni\textsuperscript{2+} inhibition of T-type channel currents were derived by fitting the data using a Hill equation: $B = 1/(1 + \textrm{IC}_{50}/[\textrm{Ni}^{2+}]^n)$, where $B$ is the normalized block, $\textrm{IC}_{50}$ is the concentration of $\textrm{Ni}^{2+}$ giving half maximal blockade, and $n$ is the Hill coefficient.   
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