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We next visualized the projections of DmCa\textsubscript{v}3-expressing neurons using another knock-in allele, \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}}.  In \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}}, the Gal4 coding sequence replaces the first coding exon of DmCa\textsubscript{v}3 and its flanking introns to drive GAL4 expression under the control of the endogenous DmCa\textsubscript{v}3 promoter (Fig. \ref{fig:3}a).  We visualized the projections of DmCa\textsubscript{v}3-positive neurons by using DmCa\textsubscript{v}3\textsuperscript{Gal4} to drive a membrane-tethered mCherry marker. marker (UAS-mChRFP).  Consistent with \emph{GFP::DmCa\textsubscript{v}3}, \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} showed broad expression pattern in the brain (Fig. \ref{fig:S1}a. We observed strong co-localization of the \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}}-driven mCherry signal and \emph{GFP::DmCa\textsubscript{v}3} in the neuropils (\ref{fig:S1}b and c}, suggesting both expressions are driven by the same endogenous DmCa\textsubscript{v}3 promoters.      
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