deletions | additions       diff --git a/Methods_sleep.tex b/Methods_sleep.tex  index 592b64c..1646fb8 100644  --- a/Methods_sleep.tex  +++ b/Methods_sleep.tex 
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For experiments using the GeneSwitch technique, flies were maintained on normal food containing 500 $\mu$M RU486 (M8046, Sigma-Aldrich) dissolved in ethanol (1\%) for two days prior to the experiment.  Control flies were maintained on normal food containing only ethanol (1\%).  During the GeneSwitch experiments, flies were placed in 2\% agar / 5\% sucrose food with or without 500 $\mu$M RU486.  For sleep deprivation experiment, activity monitors with 3-5 day-old female flies were placed in the apparatus designed to rotate and give a mechanical stimuli about twice a per  minute. After three days of 12 hr:12 hr light-dark cycle in the apparatus, files were sleep-deprived for 24 hr and recovered for following 12 hr (light period). To calculate percentage of sleep recovered (\% $\Delta$Sleep), baseline sleep (sleep during light period before the deprivation day) was subtracted from recovery sleep and then this value was divided by sleep lost.  We only included the flies withdeprivation percentage  $\geq$ 70. 70\% of sleep lost and confirmed averaged sleep lost of each genotype was about 90\%.  For the circadian locomotor analyses, we measured the activity of 1-3 day-old male flies in 30 minute bins and analyzed the data using ClockLab (Actimetrics) and the Counting Macro\cite{pfeiffenberger:2010aa}. Significance level for the $\chi$\textsuperscript{2} periodogram was set to $\alpha$ = 0.05.  Flies with a power of significance (P-S) $\geq$10 were considered rhythmic.