deletions | additions       diff --git a/Methods_sleep.tex b/Methods_sleep.tex  index b03cbd2..c1b4cb6 100644  --- a/Methods_sleep.tex  +++ b/Methods_sleep.tex 
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\subsection*{Sleep and circadian assays} locomotor behavior analysis}  Sleep and locomotor behavior of flies were measured using Drosophila Activity Monitoring system (Trikinetics).  For sleep analysis,  3-4 old female  flies were placed into 65 mm X 5mm glass tube individually with one end filled with 2\% agar/ 5\% sucrose food and the other end capped by cotton ball. Activity is counted every bim break in 1 min bin. bin and  5 minutes and more inactivity were counted as sleep conventionally. \cite{10710313}.  After one day of habituation in the incubator, incubator ($25\,^{\circ}\mathrm{C}$, 60\% humidity)  sleep during two days of 12hr:12hr light-dark cycle and following two days of dark period was analysed. analysed using Counting Macro \cite{21041393}.  Circadian locomotion was measured every 30 min bin and analysed using ClockLab analysis software (Actimetrics) with the significance level of the $\chi$\textsuperscript{2} periodogram set to $\alpha$ = 0.05.  Flies with a $\chi$\textsuperscript{2} statistic $\geq$10 over the significance line were scored as rhythmic. 
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