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\subsection*{Sleep and locomotor behavior analysis}  Sleep and locomotor behavior of flies were measured using Drosophila Activity Monitoring system (Trikinetics).  For sleep analysis, 3-4 old day-old  female flies were placed into 65 mm X 5mm glass tube individually with one end filled with 2\% agar/ 5\% sucrose food and the other end capped by cotton ball. Activity is counted was monitored as  every bim break in 1 min minute  bin and 5 minutes and more inactivity were counted as sleep \cite{10710313}. After one day of habituation in the incubator ($25\,^{\circ}\mathrm{C}$, 60\% humidity) sleep during two days of 12hr:12hr light-dark cycle and following two days of dark period was analysed using Counting Macro \cite{21041393}.  Circadian locomotion For circadian locomotor analysis, 1-3 day-old male flies were used. Activity  was measured every 30 min bin and analysed using ClockLab analysis software (Actimetrics) with the significance and Counting Macro\cite{21041392}. Significance  level of the $\chi$\textsuperscript{2} periodogram was  set to $\alpha$ = 0.05. Flies with a $\chi$\textsuperscript{2} statistic $\geq$10 over the significance line were scored as rhythmic.