deletions | additions       diff --git a/Results_sleep.tex b/Results_sleep.tex  index a3de39a..2e65e4a 100644  --- a/Results_sleep.tex  +++ b/Results_sleep.tex 
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\subsection*{DmCa\textsubscript{v}3 mutants show increased sleep}  Since the Gal4 coding sequence inserted into \emph{DmCa\textsubscript{v}3\textsuperscript{Founder}} to produce the \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} allele included a termination sequence (Fig. \ref{fig:3}a), \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} is likely a null allele.  As expected, we were unable to detect DmCa\textsubscript{v}3 expression in the fly head lysates from  \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} flies in western blot analyses using polyclonal DmCa\textsubscript{v}3-specific antisera (Fig. \ref{fig:3}a). We did, however, detect strong DmCa\textsubscript{v}3 expression in fly lysates from \emph{w\textsuperscript{1118}} controls and from a \emph{DmCa\textsubscript{v}3\textsuperscript{Rescue}} allele in which the fragment deleted in both the \emph{DmCa\textsubscript{v}3\textsuperscript{Founder}} and \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} alleles was re-inserted (Fig. \ref{fig:3}a and b). \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} homozygote flies were viable and fertile with normal appearance and no movement defect.  In mammals, two T-type channel subtypes of a1G and a1I involve in generation of neural oscillation in NREM sleep.  It is well established that flies have sleep-like state and share conserved mechanisms with mammals although it is still not clear whether flies have distinct sleep stages. 
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