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Kyunghwa Jeong edited Methods_electrophys.tex
almost 9 years ago
Commit id: 91c2a51dcded84ad97d99f65d1bc3bb838847115
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Recordings were obtained at room temperature using an Axopatch 200A amplifier, which was connected to a computer through a Digidata 1300 A/D converter, and controlled using pCLAMP 9.2 software.
Currents were recorded in a 10 mM Ba\textsuperscript{2+} solution (in mM): 140 TEACl, 2.5 CsCl, 10 BaCl2, 1 MgCl2, 10 HEPES, 10 glucose, pH = 7.3 adjusted with TEAOH. The pipette solution contained the following (in mM): 130 CsCl, 10 HEPES, 10 EGTA, 5 MgATP, 1 NaGTP, pH = 7.4 adjusted with CsOH.
Recording pipettes were prepared from TW-150-3 capillary (World Precision Instruments, Inc., Sarasota, FL).
The pipette resistance was 2.0 $\sim$ 3.0
M. M$\Omega$. Access resistance was compensated 70-80\%{} using the compensation circuit and series resistance prediction.
Data for tail currents were filtered at 10 kHz and digitized at 20 kHz.
Peak currents and exponential fits to currents were analyzed using Clampfit software (Axon instruments, Foster City, CA, USA) and graphical presentation of the data was prepared using Prism software (GraphPad, San Diego, CA, USA).
Data are presented as mean $\pm$ s.e.m.
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