deletions | additions       diff --git a/figures/fig_S1/caption.tex b/figures/fig_S1/caption.tex  new file mode 100644  index 0000000..7cb0102  --- /dev/null  +++ b/figures/fig_S1/caption.tex 
...
\label{fig:S1}  \textbf{Generation of DmCa\textsubscript{v}3 targeting alleles.}  \\  \textbf{A.} Schematic representation of DmCa\textsubscript{v}3 gene locus.  Coding exons are shown in red and start codon in 4th exon is shown.  \textbf{B.} Gene targeting by homologous recombination.  About 2kb of genomic region including the 4th exon with flanking introns was replaced by targeting construct containing attP sequence for site-specific recombination and loxP-flanked white+ marker.  White+ marker was removed by Cre-recombinase for further modification.  \textbf{C.} Schematics of DmCa\textsubscript{v}3\textsuperscript{Gal4}, DmCa\textsubscript{v}3\textsuperscript{Rescue} and GFP::DmCa\textsubscript{v}3 alleles.  Modified sequences for each alleles were inserted in integration vector (pGE-attB) carrying attB site, then integrated into attP site of DmCa\textsubscript{v}3\textsuperscript{Founder} line through $\phi$C31-mediated DNA integration.  DmCa\textsubscript{v}3\textsuperscript{Gal4} has Gal4 coding sequence flanked by splicing acceptor (SA) in 5' upstream and polyA termination sequence (pA) in 3' downstream.  In DmCa\textsubscript{v}3\textsuperscript{Rescue}, deleted exons and flanking introns of DmCa\textsubscript{v}3\textsuperscript{Founder} were reintroduced.  GFP:: DmCa\textsubscript{v}3 allele was generated by inserting multi-tags (EGFP-FlAsH-StrepII-3xFlag) and following linker sequence (GlyGlySer)4 after the ATG start codon of 4th exon in DmCa\textsubscript{v}3\textsuperscript{Rescue} construct in pGE-attB and integrating the vector into founder line.  Vector sequences including white marker was removed by Cre recombinase, leaving one loxP site.  diff --git a/figures/fig_S1/fig_S1.png b/figures/fig_S1/fig_S1.png  new file mode 100644  index 0000000..5296b26  Binary files /dev/null and b/figures/fig_S1/fig_S1.png differ diff --git a/figures/fig_S1/size.tex b/figures/fig_S1/size.tex  new file mode 100644  index 0000000..d638dc3  --- /dev/null  +++ b/figures/fig_S1/size.tex 
...
width=800  diff --git a/figures/fig_S2/caption.tex b/figures/fig_S2/caption.tex  new file mode 100644  index 0000000..96467b2  --- /dev/null  +++ b/figures/fig_S2/caption.tex 
...
\label{fig:S2}  \textbf{Costaining of GFP::DmCa\textsubscript{v}3 and DmCa\textsubscript{v}3\textsuperscript{Gal4} in adult brain.}  \\  \textbf{(A1-3)} Expression of GFP-tagged DmCa\textsubscript{v}3 and DmCa\textsubscript{v}3\textsuperscript{Gal4}-driven membrane-targeted mCherry (mCD8-ChRFP) were colocalized throughout the brain in GFP::DmCa\textsubscript{v}3/ DmCa\textsubscript{v}3\textsuperscript{Gal4} ;;UAS-mCD8-ChRFP.  Maximum intensity projections of GFP-tagged DmCa\textsubscript{v}3 (A1), mCD8-ChRFP (A2) and merged image (A3).  \textbf{(B1-3)} Expression in ellipsoid body, fan-shaped body and noduli of central complex are shown.  Scale bars, 100 $\mu$m in \textbf{A.} and 20 $\mu$m in \textbf{B.}.  diff --git a/figures/fig_S2/fig_S2.png b/figures/fig_S2/fig_S2.png  new file mode 100644  index 0000000..e036732  Binary files /dev/null and b/figures/fig_S2/fig_S2.png differ diff --git a/figures/fig_S2/size.tex b/figures/fig_S2/size.tex  new file mode 100644  index 0000000..d638dc3  --- /dev/null  +++ b/figures/fig_S2/size.tex 
...
width=800  diff --git a/figures/fig_S3/caption.tex b/figures/fig_S3/caption.tex  new file mode 100644  index 0000000..905321c  --- /dev/null  +++ b/figures/fig_S3/caption.tex 
...
\label{fig:S3}  \textbf{Deletion mutants of DmCa\textsubscript{v}3 increases sleep in constant darkness.}  \\  \textbf{A.} Schematic representation of DmCa\textsubscript{v}3 gene locus and deleted region in mutants, $\Delta$3, $\Delta$115 and $\Delta$135.  G1047 line was used for P-element imprecise excision.  Exons are denoted by black squares.  Bracketed area represents deleted regions and deletion size are indicated within the brackets.  \textbf{B.} Western blot analysis of DmCa\textsubscript{v}3 protein levels in w\textsuperscript{1118}, $\Delta$3, $\Delta$115 and $\Delta$135.  DmCa\textsubscript{v}3 protein is not detectable in all deletion mutants.  $\beta$-actin was used for internal control.  \textbf{C.} Sleep profiles of w\textsuperscript{1118} (n=31), $\Delta$3 (n=31), $\Delta$115 (n=32) and transhetero mutant $\Delta$3/$\Delta$115 (n=32) over two days of 12h:12h light-dark cycle (LD) and two days of continuous dark condition (DD).  Sleep in 30 minute intervals was plotted.  White, black and gray bar denote light phase, dark phase and subjective light phase, respectively.  ZT, zeitgeber time.  CT, circadian time.  \textbf{D.} Averaged sleep amount in light (L)/dark phase (D) in two days of LD (left) and subjective light (SL)/subjective dark (SD) phase in two days of DD (right) in DmCa\textsubscript{v}3 mutants.  w\textsuperscript{1118} (n=31), $\Delta$3 (n=31), $\Delta$115 (n=32), $\Delta$135 (n=29), $\Delta$3/$\Delta$115 (n=32), $\Delta$3/$\Delta$135 (n=32), and $\Delta$115/$\Delta$135 (n=30).  Data are represented as mean $\pm$ sem.  For statistical analysis, Mann-Whitney U test was performed.  *$p\le$0.05.  diff --git a/figures/fig_S3/fig_S3.png b/figures/fig_S3/fig_S3.png  new file mode 100644  index 0000000..d689cd3  Binary files /dev/null and b/figures/fig_S3/fig_S3.png differ diff --git a/figures/fig_S3/size.tex b/figures/fig_S3/size.tex  new file mode 100644  index 0000000..d638dc3  --- /dev/null  +++ b/figures/fig_S3/size.tex 
...
width=800  diff --git a/figures/fig_S4/caption.tex b/figures/fig_S4/caption.tex  new file mode 100644  index 0000000..fd8ee22  --- /dev/null  +++ b/figures/fig_S4/caption.tex 
...
\label{fig:S4}  \textbf{DmCa\textsubscript{v}3\textsuperscript{Gal4}-driven knock-down of DmCa\textsubscript{v}3 increases sleep in constant darkness.}  \\  \textbf{A.} Sleep profiles of w\textsuperscript{1118} (n=), DmCa\textsubscript{v}3\textsuperscript{Gal4}/+ (n=63), UAS- DmCa\textsubscript{v}3 RNAi/+ (n=63) and DmCa\textsubscript{v}3\textsuperscript{Gal4}> DmCa\textsubscript{v}3 RNAi (n=59) over two days of 12h:12h light-dark cycle (LD) and four days of continuous dark condition (DD).  Sleep in 30 minute intervals was plotted.  White, black and gray bar denote light phase, dark phase and subjective light phase, respectively.  ZT, zeitgeber time.  CT, circadian time.  \textbf{B.} Quantification of averaged sleep.  Data are represented as mean $\pm$ sem.  For statistical analysis, Mann-Whitney U test was performed.  ***$p\le$0.001.  diff --git a/figures/fig_S4/fig_S4.png b/figures/fig_S4/fig_S4.png  new file mode 100644  index 0000000..3f10c33  Binary files /dev/null and b/figures/fig_S4/fig_S4.png differ diff --git a/figures/fig_S4/size.tex b/figures/fig_S4/size.tex  new file mode 100644  index 0000000..d638dc3  --- /dev/null  +++ b/figures/fig_S4/size.tex 
...
width=800  diff --git a/figures/fig_S5/caption.tex b/figures/fig_S5/caption.tex  new file mode 100644  index 0000000..9218a8b  --- /dev/null  +++ b/figures/fig_S5/caption.tex 
...
\label{fig:S5}  \textbf{}  \\  Averaged sleep amount over two days of 12hr:12hr light-dark cycle (LD) in various neuronal Gal4-driven knock-down lines.  PI, pars intercerebralis, MB, mushroom body.  Data are represented as mean $\pm$ s.e.m.  For statistical analysis, Mann-Whitney U test was performed.  *$p\le$0.05.  diff --git a/figures/fig_S5/fig_S5.png b/figures/fig_S5/fig_S5.png  new file mode 100644  index 0000000..da6244f  Binary files /dev/null and b/figures/fig_S5/fig_S5.png differ diff --git a/figures/fig_S5/size.tex b/figures/fig_S5/size.tex  new file mode 100644  index 0000000..d638dc3  --- /dev/null  +++ b/figures/fig_S5/size.tex 
...
width=800