deletions | additions       diff --git a/Methods_IHC.tex b/Methods_IHC.tex  index 6df6b76..1659d8e 100644  --- a/Methods_IHC.tex  +++ b/Methods_IHC.tex 
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Fixed brain samples were washed with PAT3 solution (0.5\% TritonX-100, 0.5\% BSA in PBS) for 15 min 3 times and incubated in 5\% normal goat serum for 2-3 hours at room temperature.  After blocking, samples were incubated with primary antibodies diluted in 5\% normal goat serum overnight at $4\,^{\circ}\mathrm{C}$.  The samples were then washed with PAT3 for 1 hour 2 times at room temperature and incubated with secondary antibodies diluted in 5\% normal goat serum overnight at $4\,^{\circ}\mathrm{C}$.  After washing off the secondary antibodies with PAT3 for 1 hour 2 times, the brain samples were mounted in Vectashield (H-1000, Vector Laboratories, Inc.) and visualized on a LSM-XXX LSM-780  confocal microscope (Zeiss, Germany).\textcolor{red}{What kind of confocal did you use?}  Primary antibodies: rabbit anti-GFP (1:500, A11122, Invitrogen); anti-bruchpilot monoclonal (1:50, nc82, DSHB).  Secondary antibodies: goat anti-rabbit Alexa 488 (1:300, A11008, Invitrogen); goat anti-mouse Alexa 568 (1:300, A11031, Invitrogen).