deletions | additions       diff --git a/Methods_sleep.tex b/Methods_sleep.tex  index d45b838..d6ddef2 100644  --- a/Methods_sleep.tex  +++ b/Methods_sleep.tex 
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Activity was monitored as every bim break in 1 minute bin and 5 minutes and more inactivity were counted as sleep \cite{10710313}.  After one day of habituation in the incubator (25$\,^{\circ}\mathrm{C}$, 60\% humidity), sleep during two days of 12hr:12hr light-dark cycle and following two days of dark period was analysed using Counting Macro \cite{21041393}.  For circadian locomotor analysis, 1-3 day-old male flies were used. Activity was measured every 30 min bin and analysed using ClockLab analysis software (Actimetrics) and Counting Macro\cite{21041392}. Significance level of the $\chi$\textsuperscript{2} periodogram was set to $\alpha$ = 0.05.  Flies with a power of significance (P-S) $\geq$10 were scored considered  as rhythmic.