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In 10 mM Ba\textsuperscript{2+}, currents were elicited by depolarizing step pulses in 10 mV increments from \textminus70 mV to +40 mV from a holding potential of \textminus90 mV.   (Right) I-V relationships of DmCa\textsubscript{v}3 and Ca\textsubscript{v}3.1.   Peak currents for each oocyte were normalized to the maximum current recorded.   Averaged percent amplitudes (mean $\pm$ s.e.m.) from oocytes expressing DmCa\textsubscript{v}3 ($\fullmoon$) ($\circle$)  or Ca\textsubscript{v}3.1 ($\square$) are plotted against test potentials and fitted with Boltzmann equation. {\bf(b)} (Left) Steady-state inactivation was measured during voltage steps to \textminus20 mV after 10 sec prepulse to potentials between \textminus100 mV and \textminus40 mV.   (Right) Voltage-dependent activation and steady-state inactivation curves of DmCa\textsubscript{v}3 channel ($\fullmoon$, $\newmoon$) and Ca\textsubscript{v}3.1 ($\square$, $\blacksquare$) were plotted and fitted with Boltzmann equation.   {\bf(c)} The activation time constant ($\tau$\textsubscript{act}) and inactivation time constant ($\tau$\textsubscript{inact}) of DmCa\textsubscript{v}3 ($\fullmoon$) and Ca\textsubscript{v}3.1 ($\square$) were obtained by fitting the current traces with double exponentials simultaneously.   {\bf(d)} Voltage-dependent deactivation of DmCa\textsubscript{v}3 in HEK-293 cells.   Tail currents were elicited by application of repeated step pulses to \textminus20 mV for 10 ms, followed by various re-polarizing re-polarizingjavascript:void(0)  potentials from \textminus120 mV to \textminus50 mV. Deactivation time constants were obtained from fitting tail current traces with a single exponential and plotted against re-polarizing potentials (n=6).   {\bf(e)} I\textsubscript{Ca}/I\textsubscript{Ba} ratios of DmCa\textsubscript{v}3 and Ca\textsubscript{v}3.1.   (Left) Representative current traces through DmCa\textsubscript{v}3 and Ca\textsubscript{v}3.1 measured in 10 mM Ba\textsuperscript{2+} or 10 mM Ca\textsuperscript{2+} were elicited by \textminus10 mV step pulses from a holding potential of \textminus90 mV.  
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