Walton Jones results edit  almost 9 years ago

Commit id: 3567e6ad01575bd5ac6c072438de7a31db092ea0

deletions | additions      

       

During steady-state inactivation, the potentials of 50\% channel availability (V\textsubscript{50,inact}) for DmCa\textsubscript{v}3 and Ca\textsubscript{v}3.1 are estimated to be \textminus58.04 $\pm$ 0.71 and \textminus61.31 $\pm$ 0.70 mV (P $<$ 0.05, Student's t-test, n=5 -- 15). In other words, the V\textsubscript{50,inact} of DmCa\textsubscript{v}3 is 3.3 mV more positive than that of Cav\textsubscript{v}3.1 (Fig. \ref{fig:1}b and Table \ref{tab:1}).  An ion channel's so-called ``window current'' is the range of overlap in its steady-state activation and inactivation curves. This window for DmCa\textsubscript{v}3 is significantly larger than that of Ca\textsubscript{v}3.1, implying that DmCa\textsubscript{v}3 is capable of persistently passing larger currents over the relevant voltage range than Ca\textsubscript{v}3.1.  The voltage-dependent kinetics of the three mammalian T-type calcium channels are known to differ, with Ca\textsubscript{v}3.1 and Ca\textsubscript{v}3.2 showing faster activation/inactivation kinetics than Ca\textsubscript{v}3.3\cite{klockner:1999aa}.  To compare the time constants of activation and inactivation for DmCa\textsubscript{v}3 and Ca\textsubscript{v}3.1, we fitted the current traces with a double exponential function.  At test potentials ranging from \textminus50 mV to +20 mV, DmCa\textsubscript{v}3 has slower current kinetics than Ca\textsubscript{v}3.1 (P $<$ 0.01 or 0.001, Fig. \ref{fig:1}c).  For example, the activation and inactivation time constants of DmCa\textsubscript{v}3 current at a \textminus20 mV test potential are 2.2 $\pm$ 0.2 ms and 23.4 $\pm$ 1.4 ms respectively. This means the activation and inactivation kinetics of DmCa\textsubscript{v}3 are 2-fold slower than those of rat Ca\textsubscript{v}3.1 (Table \ref{tab:1}).