deletions | additions       diff --git a/sleep.tex b/sleep.tex  index 1c13bde..13d0d28 100644  --- a/sleep.tex  +++ b/sleep.tex 
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Since the Gal4 coding sequence inserted into DmCa\textsubscript{v}3\textsuperscript{Founder} to produce the DmCa\textsubscript{v}3\textsuperscript{Gal4} allele included a termination sequence (Fig. \ref{fig:S1}C), DmCa\textsubscript{v}3\textsuperscript{Gal4} is likely a null allele.  As expected, we were unable to detect DmCa\textsubscript{v}3 expression in whole fly lysates DmCa\textsubscript{v}3\textsuperscript{Gal4} flies in western blot analyses using polyclonal DmCa\textsubscript{v}3-specific antisera. We did, however, detect strong DmCa\textsubscript{v}3 expression in fly lysates from w\textsuperscript{1118} controls and from a DmCa\textsubscript{v}3\textsuperscript{Rescue} allele in which the fragment deleted in both the DmCa\textsubscript{v}3\textsuperscript{Founder} and DmCa\textsubscript{v}3\textsuperscript{Gal4} alleles was re-inserted (Fig. \ref{fig:3}A).  \todo[inline]{This comes out of nowhere.}%  DmCa\textsubscript{v}3\textsuperscript{Gal4} homozygote flies were viable and fertile with normal appearance and no movement defect.  \todo[inline]{You did much more than this. Shouldn't we at least mention some of your negative behavioral data saying data not shown?}%  In mammals, two T-type channel subtypes of a1G and a1I involve in generation of neural oscillation in NREM sleep. 
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We also generated independent deletion mutants by P-element based imprecise excision to exclude the possibility that ectopic expression of Gal4 in DmCa\textsubscript{v}3\textsuperscript{Gal4} might cause sleep phenotype.  All the deletion mutants and their trans-hetero mutants showed increased sleep in DD (Fig. \ref{fig:S3}), supporting DmCa\textsubscript{v}3 channel regulates sleep state.