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Walton Jones Fixed mention of the rescue
over 8 years ago
Commit id: 280c48289b1d00d577ffadf2349280c4c6e68472
deletions | additions
diff --git a/Results_sleep.tex b/Results_sleep.tex
index f6621b2..f11dc34 100644
--- a/Results_sleep.tex
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Flies have a well-established sleep-like state that shares some features with mammalian sleep, but it remains unclear whether flies have a stage akin to mammalian NREM sleep.
Still, we hypothesized that DmCa\textsubscript{v}3-null flies may exhibit sleep defects.
\emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} flies show increased total sleep under both 12h:12h light-dark (LD) and
constant continuous dark (DD) conditions, but this phenotype is particularly prominent during the subjective day
under in continuous
dark (DD) conditions darkness (Fig. \ref{fig:3}c and d).
Total The increase in total sleep
time observed for DmCa\textsubscript{v}3-null flies is
rescued, however, rescued in \emph{DmCa\textsubscript{v}3\textsuperscript{Rescue}}
flies to levels similar to those of \emph{w\textsuperscript{1118}} control flies flies, partially in LD conditions and completely in DD conditions (Fig. \ref{fig:3}c and d).
By The cause of this partial rescue in LD conditions is unclear.
Although the level of DmCa\textsubscript{v}3 is grossly normal in \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} flies (Fig. \ref{fig:3}b), it is possible that the addition of the \emph{attR} site and the \emph{loxP} sites in the \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} allele (Fig. \ref{fig:3}a) subtly reduce expression of DmCa~v~3 in some small but important neuronal subpopulation preventing a full rescue.
Regardless, by measuring waking locomotor activity, we were able to confirm that the increased sleep of \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} flies is not an artifact of a generalized reduction in movement.
In fact, On the contrary, \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} show slightly higher levels of waking activity than their respective controls (Fig. \ref{fig:3}e).
Normal fly sleep consists of a number of sleep bouts.
We, therefore, asked whether the increased sleep of \emph{DmCa\textsubscript{v}3\textsuperscript{Gal4}} flies is a result of an increased number of sleep bouts, prolonged bout duration, or both.