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Kyunghwa Jeong edited Results_electrophys.tex
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In steady-state inactivation, the potentials (V\textsubscript{50,inact}) of 50\% channel availability for DmCa\textsubscript{v}3 and Ca\textsubscript{v}3.1 are estimated to be \textminus58.04 $\pm$ 0.71 and \textminus61.31 $\pm$ 0.70 mV (P $<$ 0.05, Student's t-test, n=5 -- 15), indicating that the V\textsubscript{50,inact} of DmCa\textsubscript{v}3 is 3.3 mV more positive than that of Cav\textsubscript{v}3.1 (Fig. 1b and Table 1).
In regard to window current typically designated by the portion overlapped in the steady-state inactivation and activation curves, the window region for DmCa\textsubscript{v}3 is significantly larger than that for Ca\textsubscript{v}3.1, implying that DmCa\textsubscript{v}3 is capable of persistently evoking higher channel activity over relevant voltage range than Ca\textsubscript{v}3.1.
Voltage-dependent kinetics are known to different among three mammalian T-type calcium
channels7. channels\cite{10594642}.
Ca\textsubscript{v}3.1 and Ca\textsubscript{v}3.2 shows similar activation/inactivation kinetics whereas Ca\textsubscript{v}3.3 has much slower kinetics.
We compared the time constants of activation and inactivation by fitting the current traces with double exponential function.
Over At the
voltage ranges tested, test potentials from \textminus50 mV to \textplus20 mV, DmCa\textsubscript{v}3 has slower current kinetics than Ca\textsubscript{v}3.1
(P< (P $<$ 0.01 or 0.001, Fig.
1C).
DmCa\textsubscript{v}3 has 1c).
For example, the activation
kinetics of ? ms and inactivation
kinetics time constants of
? ms DmCa\textsubscript{v}3 current at
10 \textminus20 mV
potential, displaying test potential are 2.2 $\pm$ 0.2 ms and 23.4 $\pm$ 1.4 ms respectively, showing about 2-fold slower
activation and inactivation kinetics than
those of rat Ca\textsubscript{v}3.1
current (Table 1).
Previous studies have shown that the current amplitude of Ca\textsubscript{v}3.1 in Ca\textsubscript{v}3 as a charge carrier is greater than in equi-molar Ba\textsuperscript{2+}, while the opposite is true for Ca\textsubscript{v}3.2 or Ca\textsubscript{v}3.3\cite{mcrory:2000aa,shcheglovitov:2007aa}.
Herein, we determined relative permeability (ICa/IBa) of Ca\textsubscript{v}3 and Ba\textsuperscript{2+} ions through DmCa\textsubscript{v}3 or Ca\textsubscript{v}3.1.
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