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Kyunghwa Jeong edited Methods_electrophys.tex
almost 9 years ago
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Peak currents and exponential fits to currents were analyzed using Clampfit software (Axon instruments, Foster City, CA, USA).
Activation and inactivation time constants of T-type channel currents elicited by step pulses were estimated by fitting individual current traces with a double exponential function: $A1(1-exp(-t/\tau1)) + A2(1-exp(-t/\tau2))$ where $A1$ and $A2$ are the coefficients for the activation and inactivation exponentials, $t$ is time, and $\tau1$ and $\tau2$ are the activation and inactivation time constants, respectively.
The smooth curves for channel activation and steady-state inactivation were from fitting data with a Boltzmann equation: $\frac{1}{1+exp[(V_{50}-V)/S_{act}]}$ , where $V_{50}$ is the potential for half-maximal activation and $S_{act}$ is the slope conductance.
Dose-response curves for Ni\textsuperscript{2+} inhibition of T-type channel currents were derived by fitting the data using a Hill equation: $B = 1/(1 + \textrm{IC}_{50}/[\textrm{Ni}^{2+}]^n)$, where $B$ is the normalized block,
$IC_{50}$ $\textrm{IC}_{50}$ is the concentration of $\textrm{Ni}^{2+}$ giving half maximal blockade, and $n$ is the Hill coefficient.
Graphical presentation of the data was prepared using Prism software (GraphPad, San Diego, CA, USA).
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