Figure 1: Overview of approach. Poisson loading of sgRNA followed by poisson loading of cells into droplets for scalable single cell transcriptomics yields an expression matrix of  cells. By matching polyadenylated RNA barcodes to the guide RNA a perturbation matrix is resolved. By fitting a coefficient matrix for each gene and each perturbation, the transcriptome wide effect of a knockout can be mapped with high confidence.  Additional covariates such as library complexity, cell cycle, and cell state effects can also be considered using intergenic/nontargeting guides as a control.