MCF-7 cell lines were provided by and grown on Dulbecco’s modified essential medium supplemented with 10% (v/v) fetal calf serum, Penicillin (100 U/mL) and Streptomycin (100 μg/mL). Cells were seeded in 96 well plates at a concentration of 3 x 104 cells/mL of DMEM/well and incubated for 48 h at 37° C under 5% CO2 to attain confluence.