Fig 2. Protective effect of AS-IV on A549 cells infected with influenza virus. CCK8 reagent was used to detect A549 cell viability. Cell viability was defined as the percentage of the NC group. IAV, influenza A virus; NC, negative control group. * P < 0.05, ** P < 0.01.
To further evaluate the anti-influenza virus effect of AS-IV, we used the immunofluorescence method to evaluate the efficacy of AS-IV on IAV. As shown in Fig. 3A, AS-IV had no obvious inhibitory effect on the infection of the influenza virus. AS-IV also had no inhibitory effect on IAV replication (Fig. 3B-C). In addition, we also observed that AS-IV had no obvious effect on delaying the nuclear envelope shuttle transport of NP protein (Fig. 3D).