Fig 2. Protective effect
of AS-IV on A549 cells infected with influenza virus. CCK8 reagent was
used to detect A549 cell viability. Cell viability was defined as the
percentage of the NC group. IAV, influenza A virus; NC, negative control
group. * P < 0.05, ** P < 0.01.
To further evaluate the anti-influenza virus effect of AS-IV, we used
the immunofluorescence method to evaluate the efficacy of AS-IV on IAV.
As shown in Fig. 3A, AS-IV had no
obvious inhibitory effect on the infection of the influenza virus. AS-IV
also had no inhibitory effect on IAV replication
(Fig. 3B-C). In addition, we also
observed that AS-IV had no obvious effect on delaying the nuclear
envelope shuttle transport of NP protein
(Fig. 3D).