2.6. Enzyme-linked immunosorbent assay (ELISA)
After various treatments, the levels of IL-1β (Beyotime, PI305,
Shanghai, China) and IL-18 (Neobioscience, NOV-NR-E10256, Shenzhen,
China) in the cell culture supernatant were detected by specific
enzyme-linked immunosorbent assay ELISA kits based on the instructions
of the manufacturer. The value of absorbance was detected using an
enzyme-labeled instrument at 450 nm. The standard curve is made and the
measured sample concentration is calculated according to the standard
curve.