2.7 Inhibition of biofilm by the CDs
A suspension (100 μL) of S. aureus or S. typhimurium of 106 CFU/mL in concentration was added to a well from a sterile 96-well plate, to which a CD solution (100 μL) at a predetermined concentration was added. Subsequently, the mixture was exposed to UV irradiation for 30 min, and then incubated at 37°C for 48 h. Finally, the inhibitory effect of the CD on biofilm was evaluated by crystalline violet staining, as described above. The inhibitory effect of the CDs on biofilm in the dark was also evaluated under the identical conditions, except for the exemption of UV irradiation.
3 RESULTS AND DISCUSSION