2.1 Culture conditions
The biological material was the IMP-BG-036 strain, corresponding to the
marine dinoflagellate P. micans , which is part of the strain of
the Aquatic Organisms Germplasm Bank (BGOA). The cultivation was of the
batch type, for 11 days, enriched with medium FL, arranged in 250ml
glass flaks and in triplicate, submitted to a temperature of 18°C, with
photoperiod 12 h, according to experimental design factors detailed in
Table 1.
[Table 1]
The data generated was subjected to a complete factorial experiment 23,
with 3 replicates and 3 central points, completely randomized, the
treatments were shown in table 2.
[Table 2]