3.1 Growth characteristics of the P. micans strain IMP-BG-036
Figure 1 shows that the cell concentration was affected by all the factors as a whole: the volume of the inoculum, the lighting on the culture and the dose of nitrate in the medium, without there being a single selective factor. Although the standardized effects of the Pareto Diagram show that the factor with the greatest impact was the volume of the inoculum, the interaction between it and the lighting, as well as the dose of nitrate and lighting allowed the recording of the maximum cell concentrations at the end of the culture period on day 11. This result (3.1x104 cel.ml-1), was obtained with a lighting of 5 mol.m-2.sec-1, an inoculum volume of 200 ml and without nitrate dose. in the culture medium (Table 4).
[Figure 1]
[Table 4]
One aspect observed during the counting process and not considered in the design was the number of empty shells found; with higher incidence in crops whose inoculum was 200 mL. In those with a smaller volume of inoculum (50 and 125 ml), the medium was more diluted, and the valves found were very few.