2.3 Determination of lipid content
The lipid profile was determined using the Folch method (1957). 50 ml of sample was centrifuged for analysis, then 3 ml of chloroform: methanol (1:1) was added to perform lipid extraction. A Branson 2510 ultrasonic bath was used to enhance lipid extraction, the chloroform:lipid phase was separated with ultrapure water and evaporated with nitrogen gas. The result was expressed as weight percentage.