Figure 1. A monoclonal, high affinity CD8+ T
cell population generated following natural HCV infection.
A. Longitudinal measurements of viral load and IFN-γ ELISpot responses
to two HCV-derived CD8+ T cell epitopes in CL-MCRL.
IU: international units. SFU: spot-forming units. B. Representative flow
cytometry gating for Dextramer staining of HCV-specific
CD8+ T cells. C. Frequency of cells specific for the
GPR-epitope measured by Dextramer staining in CL-MCRL. D. Stacked bar
plot showing the diversity of GPR-specific clonotypes with paired CDR3α
and CDR3β amino acid sequences at time points from CL-MCRL. E. Schematic
of the koff dissociation assay using reversible
Streptamers. pMHC: peptide-MHC. F. Flow cytometry gating for the
identification of the GPR-specific population for the koff dissociation
assay. G. Dot plot of staining intensity of the reversible Streptamer
backbone and pMHC components over time. The dotted red line indicates
the time of addition of biotin. The green curve shows the non-linear
regression. The half-life of the curve was calculated and numbers in
brackets represent 95% confidence intervals. Cells were placed on ice
during intervening periods.