Figure 2. Comparative HCV-specific clonotypes from polyclonal
populations are lower affinity than the monoclonal clonotype.
A. Representative flow cytometry gating for HCV-specific
CD8+ T cell colonies stained with non-reversible
multimers. B. Representative dots plots of fluorescence intensity from
the reversible Streptamer backbone during the koffdissociation assay of single cell colony expansions. C. Representative
dots plots of fluorescence intensity from the reversible Streptamer pMHC
during the koff dissociation assay of single cell colony
expansions D. Box and whiskers plots of dissociation half-life times of
all measured clonotypes. Whiskers represent 95% confidence intervals
from non-linear regression. Pairs of solid colored symbols show cells
derived from the same colony expansion but cryopreserved and
dissociations measured independently.