Figure 2. Comparative HCV-specific clonotypes from polyclonal populations are lower affinity than the monoclonal clonotype.
A. Representative flow cytometry gating for HCV-specific CD8+ T cell colonies stained with non-reversible multimers. B. Representative dots plots of fluorescence intensity from the reversible Streptamer backbone during the koffdissociation assay of single cell colony expansions. C. Representative dots plots of fluorescence intensity from the reversible Streptamer pMHC during the koff dissociation assay of single cell colony expansions D. Box and whiskers plots of dissociation half-life times of all measured clonotypes. Whiskers represent 95% confidence intervals from non-linear regression. Pairs of solid colored symbols show cells derived from the same colony expansion but cryopreserved and dissociations measured independently.