DISCUSSION
We enriched Silphium integrifolium libraries for R genes using baits developed from the related Helianthus annuus genome to characterize the diversity across a rainfall gradient. We found that the baits recovered a substantial portion of R genes in the reference. We found geographic associations between R gene count and rainfall. Here, we discuss the Silphium R gene content across the rainfall gradient, as well as the performance of the RenSeq enrichment method, and the biological implications of the geographic pattern we uncovered.
DISEASE RESISTANCE AND RAINFALL