Tissue Collection
One ramet from each genet was grown in The Land Institute Greenhouse
until young leaves had emerged. On March 12, 2020, black plastic pots
were placed over one clone of each of the 99 plants emerging rosette and
left for 48 hours to slightly etiolate the young leaves, which improves
the quality of extracted DNA in photosynthetic active tissue containing
phenolic compounds (Dabe et al., 1993). On March 14, 3-5 unexpanded
leaves were excised from each crown. The collected tissue was placed in
a labeled envelope and immediately placed on dry ice in a cooler. Within
60 minutes of collection, the sampled leaf tissue was placed in a
lyophilizer and lyophilized for 48 hours.