Motility assays
The previously described method was employed to evaluate the inhibition
of bacterial motility on agar soft plates (Qais et al., 2019). To assess
motility, 0.5% LB agar plates were prepared with varying concentrations
of chitosan extract (10 and 100 μg/mL). A 5 µL overnight culture was
spot-inoculated at the center of each plate, which was subsequently
incubated for 18 hours. Additionally, swimming motility was evaluated
using 0.3% LB agar plates, following the same procedure. The diameter
of the swarmed or swam area was measured and reported in millimeters.