Conclusion
The current study demonstrates the successful extraction of highly
deacetylated chitosan from white shrimp (Metapenaeus affinis )
shells. Additionally, the research highlights the utility of RSM as a
valuable statistical approach for investigating the impact of various
independent parameters on chitosan preparation. These parameters include
HCl concentration, demineralization time, NaOH concentration,
deacetylation temperature, and deacetylation time. The highest degree of
deacetylation achieved in this study was 93.98%, and the purity of the
chitosan was confirmed using FTIR analysis. The extracted chitosan
exhibited remarkable antibacterial properties, along with a synergistic
effect when combined with ceftazidime. It also demonstrated anti-quorum
sensing and anti-biofilm activities against P. aeruginosa. Therefore,
the optimized chitosan extract holds great potential as a coating agent
for surgical equipment and indwelling catheters, effectively preventing
nosocomial infections caused by P. aeruginosa pathogens.
Additionally, it can find applications in the food packaging industry
and cosmetics sector.