Effect of chitosan on quorum sensing
The results demonstrated that treatment of Pseudomonas aeruginosabacteria with chitosan concentrations of 10 and 100 μg/mL led to a significant decrease in the production of pyocyanin and rhamnolipid, as well as a reduction in swimming motility and protease activity compared to the control group (P<0.05) (Fig 4). The greatest decrease was observed at the concentration of 100 μg/mL chitosan. Furthermore, treatment with chitosan resulted in a downregulation of the RhlR and LasR genes in P. aeruginosa when compared to the control group (P<0.05) (Figure 4).