Conclusion
The current study demonstrates the successful extraction of highly deacetylated chitosan from white shrimp (Metapenaeus affinis ) shells. Additionally, the research highlights the utility of RSM as a valuable statistical approach for investigating the impact of various independent parameters on chitosan preparation. These parameters include HCl concentration, demineralization time, NaOH concentration, deacetylation temperature, and deacetylation time. The highest degree of deacetylation achieved in this study was 93.98%, and the purity of the chitosan was confirmed using FTIR analysis. The extracted chitosan exhibited remarkable antibacterial properties, along with a synergistic effect when combined with ceftazidime. It also demonstrated anti-quorum sensing and anti-biofilm activities against P. aeruginosa. Therefore, the optimized chitosan extract holds great potential as a coating agent for surgical equipment and indwelling catheters, effectively preventing nosocomial infections caused by P. aeruginosa pathogens. Additionally, it can find applications in the food packaging industry and cosmetics sector.