Crystallinity
Chitosan spectra were acquired using Perkin Elmer FTIR 1600 infrared
spectroscopy instrument (Perkin Elmer Spectroscopy, USA) over a
frequency range of 4000 to 400 cm−1. To prepare the chitosan sample for
analysis, it was mixed with KBr and the resulting mixture was dried and
pressed to form a homogeneous sample/KBr disc. The absorption bands at
1655 and 3450 cm−1 were utilized to determine the degree of
deacetylation (DD%) (Kumari et al., 2015).