Fig. 5. DACA attenuates oxidative stress by regulating Nrf2 in cells. (A-C) The fluorescence analysis of DCFH-DA and GSH/GSSG in SH-SY5Y cells and primary neurons on a fluorescence plate reader at Ex/Em = 485/535 nm and Ex/Em = 490/520 nm (AC) (n = 5 per group). The analysis of SOD in SH-SY5Y cells and primary neurons is used with ELISA(AC) (n = 5 per group). Representative micrographs of DCFH-DA staining in SH-SY5Y cells (B). Scale bar 100 mm. (D-G) Immunoblot analysis of Nrf2, HO-1, GCLC and GCLM in SH-SY5Y cells and primary neurons. Data are shown as representative pictures (D, E) and quantified relative expression (F, G) (n = 5 per group). (H) Representative image of immunofluorescence staining of Nrf2 in primary neurons. Scale bar 10 mm. Data shown are mean ± SEM (n = 5); *p < 0.05, **p < 0.01 vs. control; #p < 0.05,##p < 0.01 vs. MPP+. NS. not significant.