Fig. 6. DACA maintains mitochondrial homeostasis by regulating
mitochondrial autophagy. (A-B) Representative micrographs of JC-1
staining in SH-SY5Y cells. Scale bar 100 mm. (B). The fluorescence
analysis of JC-1 in SH-SY5Y cells on a fluorescence plate reader at
Ex/Em = 514/529 nm and Ex/Em = 585/590 nm(B) (n = 5 per group). (C-D)
Immunofluorescence analysis of mitochondrial superoxide in SH-SY5Y
cells. Scale bar 100 mm. Data are shown as representative pictures(C)
and mean fluorescence intensity(D) (n = 5 per group). (E-H) Immunoblot
analysis of p62, PINK1, LC3, Bax and Bcl-2 in SH-SY5Y cells and primary
neurons. Data are shown as representative pictures (E, G) and quantified
relative expression (F, H) (n = 5 per group). Data shown are mean ± SEM
(n = 5); *p < 0.05, **p < 0.01 vs. control;#p < 0.05, ##p
< 0.01 vs. MPP+. NS. not significant.