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Aqueous Two-Phase Extraction-Assisted Distillation for Cost-Effective 2,3-Butanediol Purification
  • Pramod M Gawal
Pramod M Gawal
Indian Institute of Technology, Guwahati, The Energy and Resources Institute

Corresponding Author:[email protected]

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2,3-Butanediol (2,3-BD), known for its various applications and potential microbial production, faces challenges in commercial viability due to the high energy intensity nature of the conventional purification method. This study focuses on recovering and purifying 2,3-BD from glucose-based fermentation broth (FB) using aqueous two-phase extraction-assisted distillation (HATPED). The selected isobutanol/NaCl system studies were optimized with the parameter effect of salt, solvent, and extraction temperature on extraction efficiency of 2,3-BD through central composite design (CCD) and response surface methodology (RSM). Under Optimal conditions (30% (w/v) NaCl, 35% (v/v) isobutanol, and 40°C extraction temperature) yielded the highest partition coefficient ( KD) (6.8) and extraction efficiency (87%) of 2,3-BD exceeding the theoretical value of 86.93%, by 0.080%. The extract phase further proceeds in a distillation column to recover the 2,3-BD. In the scale-up study, the extraction and purification were conducted in two cycles, resulting in an overall 2,3-BD extraction and recovery of 97.63% and 95.88% (>99% purity), respectively. NaCl recovery from the aqueous phase reached 86.66% through evaporation. This cost-effective and scalable method provides valuable insights for developing an organic solvent/ inorganic salt ATPS-assisted distillation for 2,3-BD separation and recovery from complex fermentation broth.