Biosurfactants production
The emulsification index was determined as described previously after 48 h incubation with 50% v/v fungal spent media. Equal volumes of culture supernatant and toluene were mixed in a test tube and vortexed for 2 min. The emulsification index was calculated after 24 h using the following formula: Emulsification index (%) =(%)= [emulsion column(cm)/total column]*100. Data are from 3 biological replicates.