Growth assays on Trichoderma spent media
The effect of Trichoderma spp. spent media on S. melilotistrains was tested by monitoring bacterial growth of inocula on a
96-well microplate at 30°C for 72 hours with a microplate reader (Tecan
Infinite 200 PRO, Tecan, Switzerland). Spent media obtained from each
fungal species at 1, 2, and 3 weeks were added to inocula grown in TY
liquid medium after normalization with the fungal biomass. Relative
biomasses were calculated to the minimum dry-weight pellet. In each well
a final concentration of 50% of SM was present. For each strain-spent
medium combination, 3 independent cultures were set up and the growth
inhibition index was calculated after 72h as: 1- OD600trt/OD600 ctr, where trt are treated and ctr the control
samples.