Biosurfactants production
The emulsification index was determined as described previously after 48
h incubation with 50% v/v fungal spent media. Equal volumes of culture
supernatant and toluene were mixed in a test tube and vortexed for 2
min. The emulsification index was calculated after 24 h using the
following formula: Emulsification index (%) =(%)= [emulsion
column(cm)/total column]*100. Data are from 3 biological replicates.