3.3 Divergence Time Estimation
As we all know, the molecular markers of chloroplast are closer to neutral and have matrilineal genetic stability and widely used in the process of divergence time estimation and biogeographic deduction. We finally selected the results obtained based on common CDS for the subsequent elaboration of the differentiation history ofNotholirion . According to the beast-derived age estimate based on the common CDS of 147 plastid genomes and 4 fossil calibration sites, the crown age of Notholirion was dated to be 25.05 Ma (95% HPD:35.56-16.62 Ma). The divergence times of N. thomsonianum was estimated at 7.43 Ma (95% HPD:14.86-2.53 Ma). And The divergence ofN. macrophyllum and N. bulbuliferum occurred at 2.46 Ma (95% HPD:4.74-0.81 Ma). The results were shown in Figures 10, 11 .