Figure 3. Temperature range of three groups of Isochrysidales. Scatter plot of temperature of ice, water, and surface sediment samples where Isochrysidales was detected in environmental DNA. Blue markers represent detection of Group 2i, red and yellow markers represent absence of Group 2i where there were positive detections of Group 2w and 3, respectively. Temperature of ice and water samples arein situ temperature measured during sample collection. Surface sediment temperature are modern coldest month temperature calculated from ERA5 monthly averaged data2 over 2005-2015.