The application of Mass Spectrometry in modern Proteomics Review

Abstract

Mass spectrometry has wild application and is the function of modern proteomics
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The resolution of the mass separator depends on the m/z ratio Mass error rate

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\label{tab:matrix}Common mass analyzers specifications
Ion separation configuration Resolution Power (m/Δm) m/z range Tandem MS Turn around time
TOF 2.5 kDa - 40 k 20 - 500 kDa MS Quick
TOF/TOF 20 k 20 - 500 kDa Tandem MS Quick
Ion Mobility Q-TOF 13/40 k 40 kDa Tandem MS Quick
IT-TOF 10 k 50 - 20 k Tandem MS Quick
IT-Orbitrap 100 k 40 - 4 kDa Tandem MS Slow
Q-Orbitrap 100 k 50 - 6 kDa Tandem MS Medium
FT-ICR 200 k 10 - 10 kDa Tandem MS Slow
Ion Trap Around 1 k 50 - 4 kDa Tandem MS Medium