Cell size measurements
Strains were grown from frozen glycerol stocks overnight in 5 mL liquid YNB complete medium, and diluted using a 50X dilution factor. Cell photos were taken with a compound microscope (LeicaTM) at 100X magnification connected to a DSRL camera. Between 69 and 364 cells were measured per species. Photos were processed using the StarDist plugin for ImageJ/Fiji software, a cell detection method for microscopy images, and processed using ImageJ/Fiji’s ‘find edges’ option. We used the detection model DSB 2018
settings at 80 for probability/score threshold and 70 for overlap threshold. Cell area was measured and log transformed and a mixed model using ‘species’ as fixed effect and ‘strain’ as random effect was used on cell size as a response variable in JMP (v17.2.0).