Yeast species significantly differ in cell size
We observed significant differences in average cell size (log cell area in µm2) between species (mixed model output for fixed effect ‘species’: F7 = 14.17, p < 0.0001; Figure 3). Especially S. arboricola , S. eubayanus, andS. uvarum cells are significantly smaller than the cells of all other species, while S. cerevisiae and S. paradoxus have significantly larger cells than most other species (Figure 3). The random effect ‘strain’ explained a small (7.1%) but significant proportion of the variance in cell size (Wald p = 0.041, Table S3). Indeed, most species showed some significant differences in cell size between strains (ANOVA for all species/t-test for S. jurei : all F/t > 3.40, all p < 0.04), except for the three S. arboricola strains, which were more uniform in size.