FIGURE LEGENDS
Fig. 1 Simultaneous detection and differentiation of type II and IV PPRV through melting curve analysis by one pair of primer.A, B Standard curves of real-time PCR for type II and IV PPRV. The tenfold diluted standard plasmid of type II and IV PPRV tested with the same pair of primer. Regression lines between the Ct values and the input concentrations of type II PPRV (A) and type IV PPRV (B) plasmid DNA using SYBR Green I real-time PCR. C Melting curve analysis of type II and IV PPRV. Amplification melting curve and melting temperature values (Tm) after SYBR Green I real-time PCR followed by melting curve analysis of type II and IV PPRV. D Specificity of the real-time PCR assay. The specific fluorescent signals were detected from cDNA of type II PPRV and type IV PPRV plasmids, and the dissociation curves showed that there were two specific product peaks for type II and IV PPRV, respectively, but no specific amplification for negative control (ORFV, GTPV, and FMDV).
Table 1 Calculation the melting temperature in qRT-PCR amplified region of all the PPRV lineage II and IV strains