FIGURE LEGENDS
Fig. 1 Simultaneous detection and differentiation of type II
and IV PPRV through melting curve analysis by one pair of primer.A, B Standard curves of real-time PCR for type II and IV PPRV.
The tenfold diluted standard plasmid of type II and IV PPRV tested with
the same pair of primer. Regression lines between the Ct values and the
input concentrations of type II PPRV (A) and type IV PPRV (B) plasmid
DNA using SYBR Green I real-time PCR. C Melting curve analysis
of type II and IV PPRV. Amplification melting curve and melting
temperature values (Tm) after SYBR Green I real-time PCR followed by
melting curve analysis of type II and IV PPRV. D Specificity of
the real-time PCR assay. The specific fluorescent signals were detected
from cDNA of type II PPRV and type IV PPRV plasmids, and the
dissociation curves showed that there were two specific product peaks for
type II and IV PPRV, respectively, but no specific amplification for
negative control (ORFV, GTPV, and FMDV).
Table 1 Calculation the melting temperature in qRT-PCR
amplified region of all the PPRV lineage II and IV strains