GROWTH SURVEY
For the growth survey, seeds of 20 populations selected from 72 populations Tsushima, and 37 populations in western Kyushu except Tsushima, were examined.
After sowing in April 2011, they were grown under natural sunlight in a greenhouse.
First, 50 grains of each population were sown on filter paper containing GA3 in a Petri dish, and the humidity was kept at 25℃.
After approximately 1 ~ 2 weeks, germinated plants were transferred to cell trays and maintained in shaded cultivation tunnels.
After confirming 4 ~ 6 leaves after about 1 month, they were put in No. 2.5 pot using a mixed culture soil of red soil: peat moss: pearlite = 7:2:1.
Futhermore, after 8 ~ 12 leaves were confirmed, a maximum of 12 plants in each group were placed in No. 7 pot and managed until measurement.
In order to get close to the local environment, basal fertilizer was not used, Hyponex (N-P-K = 6 -10 -5, Hyponex Japan) which was diluted 2000 times in pots No. 2.5 and No. 7, was given once a month from 2 weeks to 1 month as additional fertilizer.
In August and October, one grain each of the promic tablets (N-P-K = 12-12-12, Hyponex Japan) was placed in each pot and given as fertilizer.
Measurements were started when flowering began from September to October.
Each measurement was performed using a digital caliper and a tape measure.
As measurement items, 36 items were set.
At first, flowering was defined the stage that the corolla of first flower completely opened and calculated the days from sowing to number of days to flowering.
Then, two flowers were randomly selected for each individual in each population, and seven floral morphology items were investigated.
Those items were length of corolla, stamen, pistil, calyx and peduncle, and width of corolla and peduncle.
The width of corolla was measured The width of corolla was measured from the upper part of the flower to the front of the lip in the longitudinal direction.
The stamens and pistils were measured by disassembling each flower.
Eight leaf morphologies were investigated on one leaf at the 10th stage-which had grown to mature enough in each individual.
The investigation items were length of leaf, petiole, and width of leaf, petiole, number of sawtooth, Depth of sawtooth lacinia, angle of leaf apex, angle between main stem and petiole.
The depth of the sawtooth was measured as the distance between the tip of the fifth sawtooth and the front part of the sawtooth.
The angle of leaf apex was measured as the angle between the leaf apex and the fifth sawtooth, and the angle of main stem and petiole was measured as the angle on the adaxial side of the stem and petiole.
When all florets of the first inflorescence of each individual fully flowered, 9 items of plant shape at flowering were investigated.
The investigation items were the number of florets, the length and the width of the flower cluster, the length of intercluster, the distance from the ground to the first flower cluster, the first flowering nodal position, the height of plant at flowering, the width of plant and the length of internode.
The number of florets, the length and the width of the flower cluster were measured using the first flower cluster of the main stem.
The distance from the first flower cluster to the second flower cluster was measured for intercluster length, and the distance from the first flower cluster to the lower node was measured for internode length.
The number of days from flowering was calculated as the flowering period when all flowers of each plant were completely closed, and 9 items of plant form at the time of closing flowers were investigated.
The investigation items were the number of flower clusters on the main stem, the height of plant at setting, the number of flowering branches, and the width of main stem.
Three items of flower color and three items of leaf color were investigated using a spectrophotometer CM -700 d (Konica MINOLTA Inc.).
The investigation items were flower and leaf color value of L*, a*, b* using Hunter Lab values.
Leaf color was measured during leaf morphometry, and flower color was measured on the lip of fresh flowers from immediately after to 1 day after anthesis.
Seven items related to flower shape, such as corolla length, and 13 items related to plant shape, such as plant height and plant width, as well as days to flowering and flowering period, were investigated for 10 individuals in each population.
Eight items related to leaves, such as leaf height, and 6 items related to colors, such as flower color, were measured for 5 individuals in each group.
Nine additional secondary variables were added to these items, and a total of 45 items were compared in each group.