GROWTH SURVEY
For the growth survey, seeds of 20 populations selected from 72
populations Tsushima, and 37 populations in western Kyushu except
Tsushima, were examined.
After sowing in April 2011, they were grown under natural sunlight in a
greenhouse.
First, 50 grains of each population were sown on filter paper containing
GA3 in a Petri dish, and the humidity was kept at 25℃.
After approximately 1 ~ 2 weeks, germinated plants were
transferred to cell trays and maintained in shaded cultivation tunnels.
After confirming 4 ~ 6 leaves after about 1 month, they
were put in No. 2.5 pot using a mixed culture soil of red soil: peat
moss: pearlite = 7:2:1.
Futhermore, after 8 ~ 12 leaves were confirmed, a
maximum of 12 plants in each group were placed in No. 7 pot and managed
until measurement.
In order to get close to the local environment, basal fertilizer was not
used, Hyponex (N-P-K = 6 -10 -5, Hyponex Japan) which was diluted 2000
times in pots No. 2.5 and No. 7, was given once a month from 2 weeks to
1 month as additional fertilizer.
In August and October, one grain each of the promic tablets (N-P-K =
12-12-12, Hyponex Japan) was placed in each pot and given as fertilizer.
Measurements were started when flowering began from September to
October.
Each measurement was performed using a digital caliper and a tape
measure.
As measurement items, 36 items were set.
At first, flowering was defined the stage that the corolla of first
flower completely opened and calculated the days from sowing to number
of days to flowering.
Then, two flowers were randomly selected for each individual in each
population, and seven floral morphology items were investigated.
Those items were length of corolla, stamen, pistil, calyx and peduncle,
and width of corolla and peduncle.
The width of corolla was measured The width of corolla was measured from
the upper part of the flower to the front of the lip in the longitudinal
direction.
The stamens and pistils were measured by disassembling each flower.
Eight leaf morphologies were investigated on one leaf at the 10th
stage-which had grown to mature enough in each individual.
The investigation items were length of leaf, petiole, and width of leaf,
petiole, number of sawtooth, Depth of sawtooth lacinia, angle of leaf
apex, angle between main stem and petiole.
The depth of the sawtooth was measured as the distance between the tip
of the fifth sawtooth and the front part of the sawtooth.
The angle of leaf apex was measured as the angle between the leaf apex
and the fifth sawtooth, and the angle of main stem and petiole was
measured as the angle on the adaxial side of the stem and petiole.
When all florets of the first inflorescence of each individual fully
flowered, 9 items of plant shape at flowering were investigated.
The investigation items were the number of florets, the length and the
width of the flower cluster, the length of intercluster, the distance
from the ground to the first flower cluster, the first flowering nodal
position, the height of plant at flowering, the width of plant and the
length of internode.
The number of florets, the length and the width of the flower cluster
were measured using the first flower cluster of the main stem.
The distance from the first flower cluster to the second flower cluster
was measured for intercluster length, and the distance from the first
flower cluster to the lower node was measured for internode length.
The number of days from flowering was calculated as the flowering period
when all flowers of each plant were completely closed, and 9 items of
plant form at the time of closing flowers were investigated.
The investigation items were the number of flower clusters on the main
stem, the height of plant at setting, the number of flowering branches,
and the width of main stem.
Three items of flower color and three items of leaf color were
investigated using a spectrophotometer CM -700 d (Konica MINOLTA Inc.).
The investigation items were flower and leaf color value of L*, a*, b*
using Hunter Lab values.
Leaf color was measured during leaf morphometry, and flower color was
measured on the lip of fresh flowers from immediately after to 1 day
after anthesis.
Seven items related to flower shape, such as corolla length, and 13
items related to plant shape, such as plant height and plant width, as
well as days to flowering and flowering period, were investigated for 10
individuals in each population.
Eight items related to leaves, such as leaf height, and 6 items related
to colors, such as flower color, were measured for 5 individuals in each
group.
Nine additional secondary variables were added to these items, and a
total of 45 items were compared in each group.