3.4PKD1 Attenuates Oxidative Stress in random skin flap
In skin flap necrosis development, oxidative stress is a critical factor. We used IHC and Western blot to determine oxidative stress-related proteins. According to IHC results, the PKD1 group showed a significantly higher SOD1 level in the dermis compared to the control group (control vs. PKD1, p<0.01; control vs. CID755673, p<0.01) (Figure 5 A, B ). We also measured eNOS, SOD1, and HO1 expression levels in the flaps by western blot. In the PKD1 group, these proteins expression was significantly upregulated than the control group, while all the above lowest results were observed in the CID755673 group (SOD1, HO1, and eNOS: control vs. PKD1, p<0.01; control vs. CID755673, p<0.01)