Reagents and antibodies
PKD1 (PRKD1; purity ≥98%) was provided by Carna Biosciences, Inc(USA). The CID755673 was purchased from MedChemExpress LLC (USA). Pentobarbital sodium, hematoxylin and eosin staining kit, lead oxide-gelatin, and diaminobenzidine (DAB) were procured from Solarbio life Science (China). Wuhan Boster Biological Technology, Ltd. and Bioget Technology provided anti-cadherin 5 and anti-GAPDH monoclonal antibodies, respectively. The Proteintech Group (Chicago, USA) provided primary antibodies against GAPDH, HO1, SOD1, MMP9, VEGF, and CASP3. Cell Signaling Technologies (Beverly, Massachusetts) provided antibodies against CYC, eNOS, cadherin 5, and Bax. Santa Cruz Biotechnology Inc. antibody (Dallas, Texas, USA) provided the second goat anti-rabbit IgG. The ECL Plus Reagent kits and the BCA Kits were obtained from PerkinElmer Life Sciences and Beyotime Biotechnology accordingly.