Tissue edema evaluation
To determine tissue edema degree, we measured the water content of the
tissue. On the seventh day after surgery and from each group, six skin
flaps were weighed, deposited at 50°C in an autoclave for dehydration,
and re-weighed until a constant weight was maintained for 48 hours at
least. ([wet weight - dry weight]/wet weight) ×100% = water content
percentage (%).