Lead Oxide Angiography
The rats (n=6) were injected with lead oxide gelatin (80mL/kg) through
the carotid artery on the seventh POD until the limbs became orange. The
perfused rats were then refrigerated at -80°C overnight. The skin flaps
were totally removed, and the blood vessels were seen using an X-ray
machine (54kVp, 40mA, 100s exposure) following thawing in the next day.