3.3 PKD1 Attenuates Apoptosis in random skin flap
Skin flap necrosis is associated with apoptosis. Thus, we used
immunohistochemistry to determine the level of CASP3 and found that
CASP3 staining was significantly reduced in the dermis of PKD1-treated
rats than the control group. A significantly higher prevalence was noted
in the CID755673 group (control vs PKD1, p <0.01; control vs
CID755673, p <0.01) (Figure 4 A, B) . The underlying
results were consistent with the western blot findings. Additionally,
immunoblotting for Bax and CYC revealed that the PKD1 group showed
significantly lower levels of CYC and Bax than the control group.
Similarly, the CID75567e3 were higher (CYC and CASP3: control vs PKD1,
p<0.01; control vs CID755673, p<0.05; Bax: control
vs PKD1, p<0.05; control vs CID755673, p<0.01)