3.2PKD1 improves angiogenesis in random skin flap
The vessel density was assessed by H&E staining, and CD34-positive
vessels were calculated under IHC. The results of HE were significantly
higher in the PKD1 group (128.50±3.60/mm2) compared to
the control group (76.83±4.40/mm2) (p<0.01).
In contrast, the CID755673 group (43.17±2.75/mm2)
showed lower results (P < 0.01). (Figure 3 A, B) .
The number of CD34-positive vessels was 68.63±4.54/mm2in the control group, 125.00±5.51/mm2 in the PKD1
group, and 35.50±2.09/mm2 in the CID755673 group. The
experimental and control groups demonstrated significant differences
(p<0.01), as well as the control and CID755673 groups
(p<0.05) (Figure 3 C, D) .
Adequate blood supply, as determined by angiogenesis, can increase flap
survival. As a result, immunohistochemistry and western blot were
utilized for determining the expression levels of neovascularization
markers. IHC analysis of VEGF and cadherin5 expression revealed that,
compared to the control group, PKD1 group results were significantly
higher; whereas results in the CID755673 group were significantly lower
(Figure 3 F, H) (VEGF and cadherin5: control vs. PKD1, p<0.01;
control vs. CID755673, p<0.01). Additionally, VEGF and
cadherin5 protein expression levels were increased in PKD1, as showed by
western blot analysis, while MMP9 and CID755673 protein expression
levels were decreased (VEGF, MMP9, and cadherin 5: control vs. PKD1,
p<0.05; control vs. CID755673, p<0.01)
(Figure 3 I, J ).