Figure 3:Effect of PKD1 on angiogenesis in the random-pattern skin flaps . (A) Images of H&E staining in each group (original magnification: 200x; scale bar: 50 μm). (B) Histogram of H&E-stained MVD. (C) CD34-positive vessels IHC staining in the studied groups (original magnification: 200x; scale bar: 50 µm). (D) CD34-positive vessel density in all groups. (E, G) IHC staining of VEGF and Cadherin 5 expressions in the flap in the studied groups (original magnification:200x; scale bar: 50 µm). (F, H) VEGF and Cadherin 5 optical density values in each group. (I) Western blotting analysis of Cadherin 5, MMP9, and VEGF expressions in the studied groups, with GAPDH as an internal control. (J) The optical density values of MMP9, VEGF, and Cadherin 5 in each group. *p < 0.05 and **p < 0.01, vs control group. Mean ± SEM was used for data presentation, n = 6 per group.