Reagents and antibodies
PKD1 (PRKD1; purity ≥98%) was provided by Carna Biosciences, Inc(USA).
The CID755673 was purchased from MedChemExpress LLC (USA). Pentobarbital
sodium, hematoxylin and eosin staining kit, lead oxide-gelatin, and
diaminobenzidine (DAB) were
procured from Solarbio life
Science (China). Wuhan Boster Biological Technology, Ltd. and Bioget
Technology provided anti-cadherin 5 and anti-GAPDH monoclonal
antibodies, respectively. The Proteintech Group (Chicago, USA) provided
primary antibodies against GAPDH, HO1, SOD1, MMP9, VEGF, and CASP3. Cell
Signaling Technologies (Beverly, Massachusetts) provided antibodies
against CYC, eNOS, cadherin 5, and Bax. Santa Cruz Biotechnology Inc.
antibody (Dallas, Texas, USA) provided the second goat anti-rabbit IgG.
The ECL Plus Reagent kits and the BCA Kits were obtained from
PerkinElmer Life Sciences and Beyotime Biotechnology accordingly.